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Journal articles
Biophysical Studies of the Induced Dimerization of Human VEGF Receptor 1 Binding Domain by Divalent Metals Competing with VEGF-A
Jean-François Gaucher
1
Marie Reille-Seroussi
2
Nathalie Gagey-Eilstein
2
Sylvain Broussy
2
Pascale Coric
1
Bili Seijo
1
Marie-Bernard Lascombe
1
Benoit Gautier
2
Wang-Quing Liu
2
Florent Huguenot
2
Nicolas Inguimbert
3
Serge Bouaziz
1
Michel Vidal
2, 4
Isabelle Broutin
1
Jean-François Gaucher 1
Author IdHAL : jean-francois-gaucher ORCID : https://orcid.org/0000-0002-8448-1491
Nathalie Gagey-Eilstein 2
Author
Sylvain Broussy 2
Author
Pascale Coric 1
Author IdHAL : pascale-coric ORCID : https://orcid.org/0000-0001-8484-1381
Bili Seijo 1
Author
Marie-Bernard Lascombe 1
Author
Benoit Gautier 2
Author
Wang-Quing Liu 2
Author
Florent Huguenot 2
Author
Nicolas Inguimbert 3
Author
Serge Bouaziz 1
Author IdHAL : serge-bouaziz ORCID : https://orcid.org/0000-0001-7792-8307
Michel Vidal 2, 4
Author
Isabelle Broutin 1
Author IdHAL : isabelle-broutin ORCID : https://orcid.org/0000-0001-9862-1232
1
LCRB - UMR 8015 - Laboratoire de cristallographie et RMN biologiques (Faculté des Sciences pharmacologiques et Biologiques - 4, av. de l’Observatoire 75270 PARIS CEDEX 06 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 8015 (France)
- UPD5 - Université Paris Descartes - Paris 5 : UMR 8015 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
2
COMETE - UMR 8638 - Chimie Organique, Médicinale et Extractive et Toxicologie Expérimentale (4 Av de l'Observatoire 75270 PARIS CEDEX 06 - France)
- UPD5 - Université Paris Descartes - Paris 5 : UMR 8638 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 8638 (France)
3
CRIOBE - Centre de recherches insulaires et observatoire de l'environnement (BP 1013 Papetoiai 98729 PAPETOAI - France)
- UPVD - Université de Perpignan Via Domitia (52 avenue Paul Alduy - 66860 Perpignan Cedex 9 - France)
- EPHE - École pratique des hautes études (4-14 Rue Ferrus, 75014 Paris - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : USR3278 (France)
4
PMC - UMR_S 648 - Pharmacochimie Moléculaire et Cellulaire (Faculté de Médecine 45, rue des Saints-Pères 75270 PARIS CEDEX 06 - France)
- UPD5 - Université Paris Descartes - Paris 5 : UMR_S648 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U648 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : IFR95 (France)
Abstract : Angiogenesis is tightly regulated through the binding of vascular endothelial growth factors (VEGFs) to their receptors (VEGFRs). In this context, we showed that human VEGFR1 domain 2 crystallizes in the presence of Zn 2+ , Co 2+ or Cu 2+ as a dimer that forms via metal-ion interactions and interlocked hydrophobic surfaces. SAXS, NMR and size exclusion chromatography analyses confirm the formation of this dimer in solution in the presence of Co 2+ , Cd 2+ or Cu 2+ . Since the metal-induced dimerization masks the VEGFs binding surface, we investigated the ability of metal ions to displace the VEGF-A binding to hVEGFR1: using a competition assay, we evidenced that the metals displaced the VEGF-A binding to hVEGFR1 extracellular domain binding at micromolar level.
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Journal articles
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https://hal-univ-perp.archives-ouvertes.fr/hal-01439207
Contributor : Sonja Böttger <>
Submitted on : Wednesday, January 18, 2017 - 1:16:59 PM
Last modification on : Monday, January 4, 2021 - 10:04:01 AM
Long-term archiving on: : Wednesday, April 19, 2017 - 2:07:06 PM
Contributor : Sonja Böttger <>
Submitted on : Wednesday, January 18, 2017 - 1:16:59 PM
Last modification on : Monday, January 4, 2021 - 10:04:01 AM
Long-term archiving on: : Wednesday, April 19, 2017 - 2:07:06 PM
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journal.pone.0167755.pdf
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Identifiers
- HAL Id : hal-01439207, version 1
- PUBMED : 27942001
- DOI : 10.1371/journal.pone.0167755
Collections
UNIV-PERP | CNRS | UNIV-PARIS5 | EPHE | AGROPOLIS | CRIOBE | INSERM | USPC | UNIV-PARIS | PSL
Citation
Jean-François Gaucher, Marie Reille-Seroussi, Nathalie Gagey-Eilstein, Sylvain Broussy, Pascale Coric, et al.. Biophysical Studies of the Induced Dimerization of Human VEGF Receptor 1 Binding Domain by Divalent Metals Competing with VEGF-A. PLoS ONE, Public Library of Science, 2016, 11 (12), pp.e0167755. ⟨10.1371/journal.pone.0167755⟩. ⟨hal-01439207⟩
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