Selection of DNA aptamers against penicillin G using Capture-SELEX for the development of an impedimetric sensor - Archive ouverte HAL Access content directly
Journal Articles Talanta Year : 2017

Selection of DNA aptamers against penicillin G using Capture-SELEX for the development of an impedimetric sensor

(1, 2) , (3, 2) , (1, 2) , (1, 2) , (1, 2) , (3, 2)
1
2
3

Abstract

This paper describes for the first time the selection of aptamers selective to penicillin. Aptamers were selected using a specific process called Capture-SELEX (Systematic Evolution of Ligands by Exponential Enrichment). This technique is based on the selection of DNA aptamers using penicillin G in solution while the ssDNA library is fixed on a support. One aptamer showing a good affinity to penicillin was finally selected and tested in electrochemical sensor configuration, using electrochemical impedance spectroscopy as detection technique. The developed aptasensor allowed the detection of penicillin in a wide concentration range, comprised between 0.4 and 1000µgL-1 Such performance was compatible with milk analysis, as the maximum residue limit tolerated in this matrix is 4µgL-1. The selectivity of the developed sensor was also studied, showing that the sensor was also able to bind other beta-lactam antibiotics, although with a weaker affinity. Finally the sensor was used for detection of penicillin G in milk. It was shown that a simple sample treatment with isopropanol followed by filtration was sufficient to eliminate matrix effects, allowing the determination of penicillin in milk at concentrations compatible with legislation requirements.
Not file

Dates and versions

hal-01770982 , version 1 (19-04-2018)

Identifiers

Cite

Nathalie Paniel, Georges Istamboulie, Athar Triki, Clément Lozano, Lise Barthelmebs, et al.. Selection of DNA aptamers against penicillin G using Capture-SELEX for the development of an impedimetric sensor. Talanta, 2017, 162, pp.232 - 240. ⟨10.1016/j.talanta.2016.09.058⟩. ⟨hal-01770982⟩
24 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More