Selection of DNA aptamers against penicillin G using Capture-SELEX for the development of an impedimetric sensor

Abstract : This paper describes for the first time the selection of aptamers selective to penicillin. Aptamers were selected using a specific process called Capture-SELEX (Systematic Evolution of Ligands by Exponential Enrichment). This technique is based on the selection of DNA aptamers using penicillin G in solution while the ssDNA library is fixed on a support. One aptamer showing a good affinity to penicillin was finally selected and tested in electrochemical sensor configuration, using electrochemical impedance spectroscopy as detection technique. The developed aptasensor allowed the detection of penicillin in a wide concentration range, comprised between 0.4 and 1000µgL-1 Such performance was compatible with milk analysis, as the maximum residue limit tolerated in this matrix is 4µgL-1. The selectivity of the developed sensor was also studied, showing that the sensor was also able to bind other beta-lactam antibiotics, although with a weaker affinity. Finally the sensor was used for detection of penicillin G in milk. It was shown that a simple sample treatment with isopropanol followed by filtration was sufficient to eliminate matrix effects, allowing the determination of penicillin in milk at concentrations compatible with legislation requirements.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-perp.archives-ouvertes.fr/hal-01770982
Contributor : Dorian Miler <>
Submitted on : Thursday, April 19, 2018 - 11:37:14 AM
Last modification on : Friday, June 21, 2019 - 7:56:04 AM

Identifiers

Collections

Citation

Nathalie Paniel, Georges Istamboulie, Athar Triki, Clément Lozano, Lise Barthelmebs, et al.. Selection of DNA aptamers against penicillin G using Capture-SELEX for the development of an impedimetric sensor. Talanta, Elsevier, 2017, 162, pp.232 - 240. ⟨10.1016/j.talanta.2016.09.058⟩. ⟨hal-01770982⟩

Share

Metrics

Record views

45