Development of an aptasensor based on a fluorescent particles-modified aptamer for ochratoxin A detection

Akhtar Hayat 1, 2 Rupesh K. Mishra 2 Gaelle Catanante 2 Jean Louis Marty 2
1 IRCBM - Interdisciplinary Research Centre in Biomedical Materials
2 BAE - Biocapteurs-Analyses-Environnement
UPVD - Université de Perpignan Via Domitia
Abstract : The presented work reports a generic fluorescent aptasensing design employing carboxy-modified fluorescent particles as a signal-generating probe and magnetic particles as a solid separation support. Carboxy-modified fluorescent particles were used to modify the aptamer and act as a signal-generating probe. Magnetic beads were used as an immobilization surface to perform the function of a solid separation support. As a proof of concept, the assay was used to detect ochratoxin A (OTA). Fluorescent detection based on the displacement and competition format was performed, and the obtained results were compared. The competition-based assays were characterized with improved analytical characteristics as compared to those based on the displacement principle. The competitive fluorescent assays showed a high sensitivity where the detection limit and IC50 were 0.005 and 7.2 nM respectively. The aptasensing platform was finally demonstrated for the detection of OTA in a beer sample. However, this is a generic approach that can be very easily extended to other matrixes to determine OTA. Additionally, the proposed concept of fluorescent particles as a signal-generating probe in combination with magnetic particles can also be integrated to other fluorescent-based affinity assays.
Keywords : Fluorescence detection Displacement/competition assays Magnetic particles Modified aptamer Ochratoxin A
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Journal articles
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https://hal-univ-perp.archives-ouvertes.fr/hal-01257030
Contributor : Olivier Savoyat <>
Submitted on : Friday, January 15, 2016 - 4:25:58 PM
Last modification on : Tuesday, November 6, 2018 - 10:46:01 AM

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Akhtar Hayat, Rupesh K. Mishra, Gaelle Catanante, Jean Louis Marty. Development of an aptasensor based on a fluorescent particles-modified aptamer for ochratoxin A detection. Analytical and Bioanalytical Chemistry, Springer Verlag, 2015, 407 (25), pp 7815-7822. ⟨10.1007/s00216-015-8952-3⟩. ⟨hal-01257030⟩

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