In vitro investigation of anticholinesterase activity of four biochemical pesticides: spinosad, pyrethrum, neem bark extract and veratrine

Abstract : The anticholinesterase activity of four biochemical pesticides: spinosad, pyrethrum, neem bark extract and veratrine was tested against one acetylcholinesterase extracted from Electrophorus electricus and two from Drosophila melanogaster. Pyrethrum, neem bark extract, and veratrine induced a dose-related inhibition of a variable type. Electric eel cholinesterase was inhibited uncompetitively by neem bark extract, the inhibition of the wilde-type fruit fly enzyme by pyrethrum was competitive, and the other enzyme-biochemical pesticide inhibitions were non-competitive. The sensitivity of each enzyme toward biochemical pesticides was variable.
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Madalina Petruta Bucur, Bogdan Bucur, Jean-Louis Marty, Gabriel-Lucian Radu. In vitro investigation of anticholinesterase activity of four biochemical pesticides: spinosad, pyrethrum, neem bark extract and veratrine. Journal of Pesticide Science, Pesticide Science Society of Japan, 2014, 39 (1), 5 p. ⟨10.1584/jpestics.D13-062⟩. ⟨hal-01174774⟩

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