Skip to Main content Skip to Navigation
New interface
Journal articles

Monitoring mRNA Half-Life in Arabidopsis Using Droplet Digital PCR

Abstract : mRNA decay is an important process in post-transcriptional regulation; in addition, it plays a crucial role in plant development and response to stress. The development of new tools to quantify mRNA decay intermediates is thus important to better characterize the dynamic of mRNA decay in various conditions. Here, we applied droplet digital PCR (ddPCR), a recent and precise PCR technology, to determine mRNA half-life in Arabidopsis seedlings. We demonstrated that ddPCR can correctly assess mRNA half-life from a wide variety of transcripts in a reproducible manner. We also demonstrated that thanks to multiplexing mRNA, the half-life of multiple transcripts can be followed in the same reaction. As ddPCR allows precise quantification, we proposed that this approach is highly suitable when a low amount of RNA is available; for the detection of many targets or for the analysis of lowly expressed transcripts.
Complete list of metadata
Contributor : Martin Rosalie Connect in order to contact the contributor
Submitted on : Thursday, November 10, 2022 - 2:12:29 PM
Last modification on : Friday, November 11, 2022 - 3:57:19 AM


Publisher files allowed on an open archive




Alexandre Boubegtitene, Rémy Merret. Monitoring mRNA Half-Life in Arabidopsis Using Droplet Digital PCR. Plants, 2022, 11 (19), pp.2616. ⟨10.3390/plants11192616⟩. ⟨hal-03847051⟩



Record views


Files downloads