The mRNA-binding proteome of a critical phase transition during Arabidopsis seed germination

Nikita Sajeev (1) , Anirban Baral (1) , Antoine H.P. America , Leo A.J. Willems (1) , Rémy Merret (2) , Leónie Bentsink (1)

1 WUR - Wageningen University and Research [Wageningen]
2 LGDP - Laboratoire Génome et développement des plantes

Arabidopsis thaliana seed germination is marked by extensive translational control at two critical phase transitions. The first transition refers to the start of hydration, the hydration translational shift. The second shift, the germination translational shift (GTS) is the phase between testa rupture and radicle protrusion at which the seed makes the all or nothing decision to germinate. The mechanism behind the translational regulation at these phase transitions is unknown. RNA binding proteins are versatile players in the post-transcriptional control of mRNAs and as such candidates for regulating translation during seed germination. Here, we report the mRNA binding protein repertoire of seeds during the GTS. Thirty seed specific RBPs and 22 dynamic RBPs were identified during the GTS, like the putative RBP Vacuolar ATPase subunit A and RBP HSP101. Several stress granule markers were identified in this study, which suggests that seeds are prepared to quickly adapt the translation of specific mRNAs in response to changes in environmental conditions during the GTS. Taken together this study provides a detailed insight into the world of RNA binding proteins during seed germination and their possible regulatory role during this developmentally regulated process

Mots clés: mRNA; RNA binding proteins; Seeds; Translation; Germination

Domaines: Botanique; Génomique, Transcriptomique et Protéomique [q-bio.GN]

Nikita Sajeev, Anirban Baral, Antoine H.P. America, Leo A.J. Willems, Rémy Merret, et al.. The mRNA-binding proteome of a critical phase transition during Arabidopsis seed germination. New Phytologist, In press, ⟨10.1111/nph.17800⟩. ⟨hal-03377537⟩