Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis - Archive ouverte HAL Access content directly
Journal Articles Plants Year : 2021

Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

(1) , (1) , (1)
1

Abstract

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to setup for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.
Fichier principal
Vignette du fichier
plants-10-00466.pdf (653.89 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03209677 , version 1 (27-04-2021)

Identifiers

Cite

Marie-Christine Carpentier, Cécile Bousquet-Antonelli, Rémy Merret. Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis. Plants, 2021, 10 (3), pp.466. ⟨10.3390/plants10030466⟩. ⟨hal-03209677⟩
72 View
87 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More