Chemical RNA Modifications: The Plant Epitranscriptome

Celso Gaspar Litholdo (1) , Cécile Bousquet-Antonelli (1)

1 LGDP - Laboratoire Génome et développement des plantes

RNA post-transcriptional modifications create an additional layer to control mRNA transcription, fate, and expression. Considering that they are non-genetically encoded, can be of reversible nature, and involved in fine-tuning gene expression, the landscape of RNA modifications has been coined the "RNA epig-enome" or "epitranscriptome." Our knowledge of the plant epitranscriptome is so far limited to 3′-uridylation and internal m 6 A and m 5 C modifications in Arabidopsis. m 6 A is the most abundant and well-studied modification on mRNAs, and involves the activities of evolutionarily conserved "writer" (methyltransfer-ase), "reader" (RNA binding proteins), and "eraser" (demethylases) proteins. In Arabidopsis, m 6 A is crucial for embryogenesis, post-embryonic growth, development , phase transition, and defense responses. Conversely to animals, our understanding of the roles of m 6 A is limited to the finding that it is an mRNA stabilizing mark. Yet likely to exist, its roles in controlling plant mRNA maturation, trafficking , storage, and translation remain unexplored. The m 5 C mark is much less abundant on the transcriptome and our knowledge in plants is more limited. Nonetheless, it is also an important epitranscriptomic mark involved in plant development and adaptive response. Here, we explore the current information on m 6 A and m 5 C marks and report knowledge on their distribution, features, and molecular, cellular , and physiological roles, therefore, uncovering the fundamental importance in plant development and acclimation of RNA epigenetics. Likely to be widespread in the green lineage and given their crucial roles in eukaryotes, the fostering of data and knowledge of epitranscriptome from cultivated plant species is of the utmost importance.

Domaines: Sciences du Vivant [q-bio]

Celso Gaspar Litholdo, Cécile Bousquet-Antonelli. Chemical RNA Modifications: The Plant Epitranscriptome. Epigenetics in Plants of Agronomic Importance: Fundamentals and Applications, Springer International Publishing, pp.291-310, 2019, ⟨10.1007/978-3-030-14760-0_11⟩. ⟨hal-02125075⟩