Ultra-sensitive biosensor based on genetically engineered acetylcholinesterase immobilized in poly (vinyl alcohol)/Fe–Ni alloy nanocomposite for phosmet detection in olive oil

A.Y. El-Moghazy 1, 2 E.A. Soliman 1 H.Z. Ibrahim 3 T. Noguer 2 J.-L. Marty 2 G. Istamboulie 2
1 Polymeric Materials Research Department
2 BAE - Biocapteurs-Analyses-Environnement
UPVD - Université de Perpignan Via Domitia
3 Department of Environmental Studies
Abstract : An ultra-sensitive screen-printed biosensor was successfully developed for phosmet detection in olive oil, based on a genetically-engineered acetylcholinesterase (AChE) immobilized in a azide-unit water-pendant polyvinyl alcohol (PVA-AWP)/Fe-Ni alloy nanocomposite. Fe-Ni not only allowed amplifying the response current but also lowering the applied potential from 80 mV to 30 mV vs Ag/AgCl. The biosensor showed a very good analytical performance for phosmet detection, with a detection limit of 0.1 nM. This detection limit is lower than the allowable concentrations set by international regulations. In addition to the good reproducibility, operational and storage stability, the developed biosensor was successfully used for the determination of phosmet in olive oil samples without any laborious pre-treatment. The phosmet recovery rate was about 96% after a simple liquid-liquid extraction.
Keywords : Acetylcholinesterase Biosensor Nanocomposite Olive oil Organophosphorus pesticides Screen-printed electrodes
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Journal articles
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https://hal-univ-perp.archives-ouvertes.fr/hal-01770994
Contributor : Dorian Miler <>
Submitted on : Thursday, April 19, 2018 - 11:46:07 AM
Last modification on : Thursday, October 10, 2019 - 2:18:01 PM

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A.Y. El-Moghazy, E.A. Soliman, H.Z. Ibrahim, T. Noguer, J.-L. Marty, et al.. Ultra-sensitive biosensor based on genetically engineered acetylcholinesterase immobilized in poly (vinyl alcohol)/Fe–Ni alloy nanocomposite for phosmet detection in olive oil. Food Chemistry, Elsevier, 2016, 203, pp.73 - 78. ⟨10.1016/j.foodchem.2016.02.014⟩. ⟨hal-01770994⟩

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