Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives

Abstract : New chemiluminescence-based immunoassays for sensitive detection of 17-β estradiol (E2) and ethinylestradiol (EE2) are described on the basis of the use of biotinylated estrogen derivatives. Estrogen derivatives bearing a carboxylic group (E2-COOH and EE2-COOH) on C-3 position were synthesized, covalently bound to aminated biotin and subsequently immobilized on avidin-coated microtiter plates. The assay principle was based on competition between free and immobilized estrogens for their binding to primary antibodies, with subsequent revelation using horseradish peroxidase (HRP)-labeled secondary antibodies. Under optimized conditions, the chemiluminescence immunoassays showed a highly sensitive response to E2 and EE2, with respective detection limits of 0.5 and 1.2 ng L-1. The LOD achieved using biotinylated E2 was in the same order of magnitude as those obtained using commercially available E2-bovine serum albumin conjugate (E2-BSA). The developed devices were successfully applied to analysis wastewater treatment plants effluents (WWTP) with negligible matrix effect.
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https://hal-univ-perp.archives-ouvertes.fr/hal-01770955
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Submitted on : Thursday, April 19, 2018 - 11:20:36 AM
Last modification on : Friday, May 24, 2019 - 5:02:42 PM

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Hussein Kanso, Nicolas Inguimbert, Georges Istamboulie, Lise Barthelmebs, Carole Calas-Blanchard, et al.. Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives. Analytical Biochemistry, Elsevier Masson, 2017, 537, pp.63 - 68. ⟨10.1016/j.ab.2017.08.023⟩. ⟨hal-01770955⟩

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