Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives

Hussein Kanso; Nicolas Inguimbert; Georges Istamboulie; Lise Barthelmebs; Carole Calas-Blanchard; Thierry Noguer

Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives

Hussein Kanso ^{1, 2} Nicolas Inguimbert ³ Georges Istamboulie ^{1, 2} Lise Barthelmebs ^{2, 1} Carole Calas-Blanchard ^{2, 1} Thierry Noguer ²

1 LBBM - Laboratoire de Biodiversité et Biotechnologies Microbiennes

2 BAE - Biocapteurs-Analyses-Environnement

UPVD - Université de Perpignan Via Domitia

3 CRIOBE - Centre de recherches insulaires et observatoire de l'environnement

Abstract : New chemiluminescence-based immunoassays for sensitive detection of 17-β estradiol (E2) and ethinylestradiol (EE2) are described on the basis of the use of biotinylated estrogen derivatives. Estrogen derivatives bearing a carboxylic group (E2-COOH and EE2-COOH) on C-3 position were synthesized, covalently bound to aminated biotin and subsequently immobilized on avidin-coated microtiter plates. The assay principle was based on competition between free and immobilized estrogens for their binding to primary antibodies, with subsequent revelation using horseradish peroxidase (HRP)-labeled secondary antibodies. Under optimized conditions, the chemiluminescence immunoassays showed a highly sensitive response to E2 and EE2, with respective detection limits of 0.5 and 1.2 ng L-1. The LOD achieved using biotinylated E2 was in the same order of magnitude as those obtained using commercially available E2-bovine serum albumin conjugate (E2-BSA). The developed devices were successfully applied to analysis wastewater treatment plants effluents (WWTP) with negligible matrix effect.

Keywords : Competition Estradiol Ethinylestradiol Immunoassay Luminescence

Document type :

Journal articles

Domain :

Chemical Sciences

Chemical Sciences / Analytical chemistry

Complete list of metadatas

https://hal-univ-perp.archives-ouvertes.fr/hal-01770955
Contributor : Dorian Miler <>
Submitted on : Thursday, April 19, 2018 - 11:20:36 AM
Last modification on : Friday, May 24, 2019 - 5:02:42 PM

Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives

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Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives

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