Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

Daphné Grulois 1, 2 Serge Planes 1, 3 Cécile Fauvelot 1, 2
1 LabEX CORAIL - Laboratoire d'Excellence CORAIL
2 IRD [Sénégal] - Institut de recherche pour le développement
3 CRIOBE - Centre de recherches insulaires et observatoire de l'environnement
Abstract : Centropyge bispinosa is one of the most frequently coral reef fish exported through the aquarium trade. We developed fifteen microsatellite markers to evaluate possible distinct breeding stocks throughout its Indo-Pacific distribution range. Number of alleles varied from 2 to 50 per locus, observed and expected heterozygosities ranged from 0.486 to 0.953 and 0.482 to 0.974 respectively. Significant deviations from HWE were detected in two loci. Cross-amplifications were tested in four congener species: C. loriculus, C. bicolor, C. flavissima and C. vroliki. The 15 new microsatellite loci will be helpful tools for the identification of breeding units, connectivity studies, as well as phylogenetic relationships among pygmy angelfishes.
Keywords : Coral reef fish Aquarium trade Indo-Pacific Genetic structure
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Journal articles
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Submitted on : Monday, July 4, 2016 - 4:24:00 PM
Last modification on : Wednesday, November 20, 2019 - 7:10:42 AM

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Daphné Grulois, Serge Planes, Cécile Fauvelot. Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners. Conservation Genetics Resources, Springer, 2015, 7 (1), p. 291-293. ⟨10.1007/s12686-014-0363-9⟩. ⟨hal-01341716⟩

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