Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

Abstract : Centropyge bispinosa is one of the most frequently coral reef fish exported through the aquarium trade. We developed fifteen microsatellite markers to evaluate possible distinct breeding stocks throughout its Indo-Pacific distribution range. Number of alleles varied from 2 to 50 per locus, observed and expected heterozygosities ranged from 0.486 to 0.953 and 0.482 to 0.974 respectively. Significant deviations from HWE were detected in two loci. Cross-amplifications were tested in four congener species: C. loriculus, C. bicolor, C. flavissima and C. vroliki. The 15 new microsatellite loci will be helpful tools for the identification of breeding units, connectivity studies, as well as phylogenetic relationships among pygmy angelfishes.