Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

Daphné Grulois; Serge Planes; Cécile Fauvelot

doi:10.1007/s12686-014-0363-9

Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

Daphné Grulois ^{1, 2} Serge Planes ^{1, 3} Cécile Fauvelot ^{1, 2}

1 LabEX CORAIL - Laboratoire d'Excellence CORAIL

2 IRD [Sénégal] - Institut de recherche pour le développement

3 CRIOBE - Centre de recherches insulaires et observatoire de l'environnement

Abstract : Centropyge bispinosa is one of the most frequently coral reef fish exported through the aquarium trade. We developed fifteen microsatellite markers to evaluate possible distinct breeding stocks throughout its Indo-Pacific distribution range. Number of alleles varied from 2 to 50 per locus, observed and expected heterozygosities ranged from 0.486 to 0.953 and 0.482 to 0.974 respectively. Significant deviations from HWE were detected in two loci. Cross-amplifications were tested in four congener species: C. loriculus, C. bicolor, C. flavissima and C. vroliki. The 15 new microsatellite loci will be helpful tools for the identification of breeding units, connectivity studies, as well as phylogenetic relationships among pygmy angelfishes.

Keywords : Coral reef fish Aquarium trade Indo-Pacific Genetic structure

Type de document :

Article dans une revue

Conservation Genetics Resources, Springer, 2015, 7 (1), p. 291-293. 〈10.1007/s12686-014-0363-9〉

Domaine :

Sciences du Vivant [q-bio] / Ecologie, Environnement

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https://hal-univ-perp.archives-ouvertes.fr/hal-01341716
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Soumis le : lundi 4 juillet 2016 - 16:24:00
Dernière modification le : mardi 16 octobre 2018 - 01:01:36

Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

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Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

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