Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

Daphné Grulois; Serge Planes; Cécile Fauvelot

Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

Daphné Grulois ^{1, 2} Serge Planes ^{1, 3} Cécile Fauvelot ^{1, 2}

1 LabEX CORAIL - Laboratoire d'Excellence CORAIL

2 IRD [Sénégal] - Institut de recherche pour le développement

3 CRIOBE - Centre de recherches insulaires et observatoire de l'environnement

Abstract : Centropyge bispinosa is one of the most frequently coral reef fish exported through the aquarium trade. We developed fifteen microsatellite markers to evaluate possible distinct breeding stocks throughout its Indo-Pacific distribution range. Number of alleles varied from 2 to 50 per locus, observed and expected heterozygosities ranged from 0.486 to 0.953 and 0.482 to 0.974 respectively. Significant deviations from HWE were detected in two loci. Cross-amplifications were tested in four congener species: C. loriculus, C. bicolor, C. flavissima and C. vroliki. The 15 new microsatellite loci will be helpful tools for the identification of breeding units, connectivity studies, as well as phylogenetic relationships among pygmy angelfishes.

Keywords : Coral reef fish Aquarium trade Indo-Pacific Genetic structure

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Ecology, environment

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https://hal-univ-perp.archives-ouvertes.fr/hal-01341716
Contributor : Olivier Savoyat <>
Submitted on : Monday, July 4, 2016 - 4:24:00 PM
Last modification on : Saturday, April 20, 2019 - 2:06:22 AM

Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

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Isolation and characterization of fifteen microsatellite loci in two-spined angelfish Centropyge bispinosa (family Pomacanthidae) with cross-amplification success in four Centropyge congeners

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