Tetramethyl-6-carboxyrhodamine quenching-based aptasensing platform for aflatoxin B1: Analytical performance comparison of two aptamers - Archive ouverte HAL Access content directly
Journal Articles Analytical Biochemistry Year : 2016

Tetramethyl-6-carboxyrhodamine quenching-based aptasensing platform for aflatoxin B1: Analytical performance comparison of two aptamers

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Abstract

In this study, a simple TAMRA (tetramethyl-6-carboxyrhodamine) quenching-based aptasensing platform was designed for the detection of aflatoxin B1 (AFB1). Here, we compared the analytical performance of two aptamer sequences: seqA and seqB. The AFB1 detection was based on the interactions of FAM (carboxyfluorescein)-labeled aptamer with TAMRA-labeled DNA complementary strand in the presence and absence of target analyte. Under optimized experimental conditions, TAMRA-labeled strand quenched the fluorescence response of FAM-labeled aptamer due to the noncovalent interaction between the two DNA strands. The binding of AFB1 induced the complex formation and weakened the interaction between FAM-labeled aptamer and TAMRA-labeled complementary strand, resulting in the fluorescence recovery. By using this principle concept, an assay was constructed for the detection of AFB1. The method exhibited good sensitivity, good selectivity with a limit of detection of 0.2 ng ml−1, and a wide linear range from 0.25 to 32 ng ml−1. For real sample application, the aptasensors were tested in beer and wine samples, with good recovery rates obtained for AFB1 detection.
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Dates and versions

hal-01341200 , version 1 (04-07-2016)

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K. Yugender Goud, Atul Sharma, Akhtar Hayat, Gaëlle Catanante, K. Vengatajalabathy Gobi, et al.. Tetramethyl-6-carboxyrhodamine quenching-based aptasensing platform for aflatoxin B1: Analytical performance comparison of two aptamers. Analytical Biochemistry, 2016, 508, pp.Pages 19-24. ⟨10.1016/j.ab.2016.05.018⟩. ⟨hal-01341200⟩

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