Two-dimensional ultra high pressure liquid chromatography quadrupole/time-of-flight mass spectrometry for semi-targeted natural compounds identification

Grégory Genta-Jouve (1) , Julie Croué (2) , Lionel Weinberg (3) , Vincent Cocandeau (3) , Serge Holderith (3) , Nataly Bontemps (4) , Marcelino Suzuki (2) , Olivier P. Thomas (5, 6)

1 UMR 8638 - Equipe Pharmacognosie
2 LOMIC - Laboratoire d'Océanographie Microbienne
3 Département Actifs, Direction Recherche & Technologie
4 CRIOBE - Centre de recherches insulaires et observatoire de l'environnement
5 ICN - Institut de Chimie de Nice
6 IMBE - Institut méditerranéen de biodiversité et d'écologie marine et continentale

Isolation of minor compounds from natural extracts remains a complicated task and development of new protocols to facilitate the access to new structure is still needed. Application of a highly efficient 2D LC hyphenated to high resolution mass spectroscopy on the crude extract of the Mediterranean marine sponge Crambe crambe led to the identification of seven new compounds. The structure elucidation has been performed using 2D LC–MS/MS and NMR. The structure elucidation of these minor metabolites encouraged us to speculate on the metabolic pathway leading to this important family of sponge alkaloids.

Keywords: Natural products chemistry; Crambe crambe; Semi-targeted metabolomics; Crambescins; 2D LC–MS

Domains: Chemical Sciences

Grégory Genta-Jouve, Julie Croué, Lionel Weinberg, Vincent Cocandeau, Serge Holderith, et al.. Two-dimensional ultra high pressure liquid chromatography quadrupole/time-of-flight mass spectrometry for semi-targeted natural compounds identification. Phytochemistry Letters, 2014, 10, pp.Pages 318-323. ⟨10.1016/j.phytol.2014.10.029⟩. ⟨hal-01333524⟩