A label free aptasensor for Ochratoxin A detection in cocoa beans: An application to chocolate industries

Rupesh K. Mishra 1 Akhtar Hayat 2, 1 Gaëlle Catanante 1 Cristina Ocaña 1 Jean-Louis Marty 1
1 BAE - Biocapteurs-Analyses-Environnement
UPVD - Université de Perpignan Via Domitia
2 IRCBM - Interdisciplinary Research Centre in Biomedical Materials
Abstract : Contamination of food by mycotoxin occurs in minute/trace quantities. Nearly 92.5% of the cocoa samples present Ochratoxin A (OTA) levels at trace quantity. Hence, there is a necessity for a highly sensitive and selective device that can detect and quantify these organic toxins in various matrices such as cocoa beans. This work reports for the first time, a facile and label-free electrochemical impedimetric aptasensor for rapid detection and quantitation of OTA in cocoa beans. The developed aptasensor was constructed based on the diazonium-coupling reaction mechanism for the immobilization of anti-OTA-aptamer on screen printed carbon electrodes (SPCEs). The aptasensor exhibited a very good limit of detection (LOD) as low as 0.15 ng/mL, with added advantages of good selectivity and reproducibility. The increase in electron transfer resistance was linearly proportional to the OTA concentration in the range 0.15–2.5 ng/mL, with an acceptable recovery percentage (91–95%, RSD = 4.8%) obtained in cocoa samples. This work can facilitate a general model for the detection of OTA in cocoa beans based on the impedimetric aptasensor. The analysis can be performed onsite with pre-constructed and aptamer modified electrodes employing a portable EIS set up.
Keywords : Aptasensor Cocoa beans Label free Impedimetric Ochratoxin Screen printed electrodes
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Journal articles
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https://hal-univ-perp.archives-ouvertes.fr/hal-01257017
Contributor : Olivier Savoyat <>
Submitted on : Friday, January 15, 2016 - 4:20:20 PM
Last modification on : Tuesday, November 6, 2018 - 10:46:01 AM

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Rupesh K. Mishra, Akhtar Hayat, Gaëlle Catanante, Cristina Ocaña, Jean-Louis Marty. A label free aptasensor for Ochratoxin A detection in cocoa beans: An application to chocolate industries. Analytica Chimica Acta, Elsevier Masson, 2015, 889, p. 106-112. ⟨10.1016/j.aca.2015.06.052⟩. ⟨hal-01257017⟩

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