Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor

Abstract : In this work, we propose for the first time a sensitive Ochratoxin A (OTA) detection in cocoa beans using competitive aptasensor by differential pulse voltammetry (DPV). In the proposed method, biotin labeled and free OTA competed to bind with immobilized aptamer onto the surface of a screen printed carbon electrode (SPCE), and percentage binding was calculated. The detection was performed after adding avidin-ALP to perform avidin–biotin reaction; the signal was generated through a suitable substrate 1-naphthyl phosphate (1-NP), for alkaline phosphatase (ALP). The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns specifically designed for OTA. The developed aptasensor showed a good linearity in the range 0.15–5 ng/mL with the limit of detection (LOD) 0.07 ng/mL and 3.7% relative standard deviation (RSD). The aptasensor displayed good recovery values in the range 82.1–85% with 3.87% RSD, thus, demonstrated the efficiency of proposed aptasensor for such matrices.
Type de document :
Article dans une revue
Food Chemistry, Elsevier, 2016, 192, p. 799-804. 〈10.1016/j.foodchem.2015.07.080〉
Liste complète des métadonnées

https://hal-univ-perp.archives-ouvertes.fr/hal-01257010
Contributeur : Olivier Savoyat <>
Soumis le : vendredi 15 janvier 2016 - 16:16:51
Dernière modification le : vendredi 15 janvier 2016 - 16:16:52

Identifiants

Collections

Citation

Rupesh K. Mishra, Akhtar Hayat, Gaëlle Catanante, Georges Istamboulie, Jean-Louis Marty. Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor. Food Chemistry, Elsevier, 2016, 192, p. 799-804. 〈10.1016/j.foodchem.2015.07.080〉. 〈hal-01257010〉

Partager

Métriques

Consultations de la notice

79