Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor

Rupesh K. Mishra 1 Akhtar Hayat 2, 1 Gaëlle Catanante 1 Georges Istamboulie 1 Jean-Louis Marty 1
1 BAE - Biocapteurs-Analyses-Environnement
UPVD - Université de Perpignan Via Domitia
2 IRCBM - Interdisciplinary Research Centre in Biomedical Materials
Abstract : In this work, we propose for the first time a sensitive Ochratoxin A (OTA) detection in cocoa beans using competitive aptasensor by differential pulse voltammetry (DPV). In the proposed method, biotin labeled and free OTA competed to bind with immobilized aptamer onto the surface of a screen printed carbon electrode (SPCE), and percentage binding was calculated. The detection was performed after adding avidin-ALP to perform avidin–biotin reaction; the signal was generated through a suitable substrate 1-naphthyl phosphate (1-NP), for alkaline phosphatase (ALP). The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns specifically designed for OTA. The developed aptasensor showed a good linearity in the range 0.15–5 ng/mL with the limit of detection (LOD) 0.07 ng/mL and 3.7% relative standard deviation (RSD). The aptasensor displayed good recovery values in the range 82.1–85% with 3.87% RSD, thus, demonstrated the efficiency of proposed aptasensor for such matrices.
Keywords : OTA Cocoa beans Food safety DPV Competition based aptasensor
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Journal articles
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https://hal-univ-perp.archives-ouvertes.fr/hal-01257010
Contributor : Olivier Savoyat <>
Submitted on : Friday, January 15, 2016 - 4:16:51 PM
Last modification on : Tuesday, November 6, 2018 - 10:46:01 AM

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Rupesh K. Mishra, Akhtar Hayat, Gaëlle Catanante, Georges Istamboulie, Jean-Louis Marty. Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor. Food Chemistry, Elsevier, 2016, 192, p. 799-804. ⟨10.1016/j.foodchem.2015.07.080⟩. ⟨hal-01257010⟩

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