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Titanium Dioxide Nanoparticles (TiO 2 ) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection

Abstract : We demonstrate for the first time, the development of titanium dioxide nanoparticles (TiO2) quenching based aptasensing platform for detection of target molecules. TiO2 quench the fluorescence of FAM-labeled aptamer (fluorescein labeled aptamer) upon the non-covalent adsorption of fluorescent labeled aptamer on TiO2 surface. When OTA interacts with the aptamer, it induced aptamer G-quadruplex complex formation, weakens the interaction between FAM-labeled aptamer and TiO2, resulting in fluorescence recovery. As a proof of concept, an assay was employed for detection of Ochratoxin A (OTA). At optimized experimental condition, the obtained limit of detection (LOD) was 1.5 nM with a good linearity in the range 1.5 nM to 1.0 µM for OTA. The obtained results showed the high selectivity of assay towards OTA without interference to structurally similar analogue Ochratoxin B (OTB). The developed aptamer assay was evaluated for detection of OTA in beer sample and recoveries were recorded in the range from 94.30%–99.20%. Analytical figures of the merits of the developed aptasensing platform confirmed its applicability to real OPEN ACCESS Toxins 2015, 7 3772 samples analysis. However, this is a generic aptasensing platform and can be extended for detection of other toxins or target analyte.
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Submitted on : Friday, January 15, 2016 - 4:01:54 PM
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Atul Sharma, Akhtar Hayat, Rupesh K. Mishra, Gaëlle Catanante, Sunil Bhand, et al.. Titanium Dioxide Nanoparticles (TiO 2 ) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection. Journal of natural toxins, 2015, 7 (9), p. 3771-3784. ⟨10.3390/toxins7093771⟩. ⟨hal-01256991⟩



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