A Simple Colorimetric Enzymatic-Assay, based on immobilization of acetylcholinesterase by adsorption, for sensitive detection of organophosphorus insecticides in olive oil

Najwa Ben Oujji 1, 2 Idriss Bakas 1, 2 Georges Istamboulié 1 Ihya Ait-Ichou 2 Elhabib Ait-Addi 2 Régis Rouillon 1 Thierry Noguer 1
1 IMAGES - Institut de Modélisation et d'Analyse en Géo-Environnement et Santé
2 AQUAMAR - Laboratoire systèmes aquatiques, Milieu marin et continental
Abstract : Over the last decades, cholinesterase (ChE) biosensors/bioassays have emerged as an ultra sensitive and rapid technique for pesticides detection in environmental monitoring, food and quality control. In this work a Simple Colorimetric Enzymatic-Assay for the sensitive detection of three organophosphorus insecticides widely used in the treatment of olive trees: Malathion, Dimethoate and Methidathion in their oxidized form was developed. The systems are based on the immobilization of 0.3 mIU of electric eel acetylcholinesterase by adsorption on MaxiSorp™ microplates, and tested using standard solutions and real samples of olive oil. The developed colorimetric tests showed good analytical performances with limits of detection as low as 10−9 M for the widely used pesticide malaoxon (oxidized malathion), as well as a good reproducibility, good operational and storage stability and they can be applied directly on olive oil real samples without a laborious pre-treatment and after a simple liquid–liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.
Keywords : Electric eel acetylcholinesterase Colorimetric Enzymatic-Assay Olive oil Organophosphorus insecticides
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Journal articles
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Submitted on : Monday, November 16, 2015 - 5:29:36 PM
Last modification on : Friday, April 19, 2019 - 4:12:26 PM

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Najwa Ben Oujji, Idriss Bakas, Georges Istamboulié, Ihya Ait-Ichou, Elhabib Ait-Addi, et al.. A Simple Colorimetric Enzymatic-Assay, based on immobilization of acetylcholinesterase by adsorption, for sensitive detection of organophosphorus insecticides in olive oil. Food Control, Elsevier, 2014, 46, p. 75-80. ⟨10.1016/j.foodcont.2014.05.006⟩. ⟨hal-01229525⟩

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