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Journal Articles Talanta Year : 2016

Development of an impedimetric aptasensor for the determination of aflatoxin M1 in milk

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Abstract

An aptasensor was designed for the determination of aflatoxin M1 (AFM1) in milk based on DNA-aptamer recognition and electrochemical impedance spectroscopy detection. A hexaethyleneglycol-modified 21-mer oligonucleotide was immobilized on a carbon screen-printed electrode through carbodiimide immobilization, after diazonium activation of the sensing surface. Cyclic voltammetry and electrochemical impedance spectroscopy in the presence of ferri/ferrocyanide redox probe were used to characterize each step of the aptasensor development. Aptamer-AFM1 interaction induced an increase in electron-transfer resistance, allowing the determination of AFM1 in buffer in the range 2–150 ng/L (LOD=1.15 ng/L). Application to milk analysis showed that a preliminary treatment was mandatory. A simple filtration through a 0.2 µm PTFE membrane allowed determination of AFM1 in milk for concentrations ranging from 20 to 1000 ng/kg. These performances are compatible with the AFM1 levels set in European Union for milk and dairy products for adults (50 ng/kg) and infants (25 ng/kg).
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Dates and versions

hal-01220620 , version 1 (26-10-2015)

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Georges Istamboulié, Nathalie Paniel, Lorena Zara, Lucia Reguillo Granados, Lise Barthelmebs, et al.. Development of an impedimetric aptasensor for the determination of aflatoxin M1 in milk. Talanta, 2016, 146, ⟨10.1016/j.talanta.2015.09.012⟩. ⟨hal-01220620⟩

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